transposition mixture (Illumina Inc)

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Illumina Inc transposition mixture
Transposition Mixture, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 990 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/transposition+mixture/Illumina+Tagment+DNA+Enzyme+and+Buffer+Small+Kit/pmc13000455-121-13-22
Average 97 stars, based on 990 article reviews

transposition mixture - by Bioz Stars, 2026-10

97/100 stars

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Article Title: Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate.
Article Snippet: .. The supernatant was removed and the nuclei pellet was resuspended in 50 μl transposition mixture [25 μl Tagment DNA Buffer (1 × final, Illumina, 20034197), 2.5 μl Tagment Tn5 Transposase (100 nM final, Illumina, 20034197), 16.5 μl 1 × PBS, 0.5 μl 1% Digitonin (0.01% final), 0.5 μl 10% tween 20 (0.1% final)] and incubated at 37 ◦C for 30 min shaking at 1000 rpm. .. The tagmented DNA was purified with the Zymo DNA Clean and Concentrator-5 Kit (Zymo, D4014) and eluted in 21 μl Elution Buffer.

Article Title: Non-canonical enhancers control gene expression and cell fate in human pluripotent stem cells
Article Snippet: .. The pellet was resuspended in 50μl of transposition mixture (1X Illumina Tagment DNA Buffer and 100nM Illumina TDE1 Tagment DNA Enzyme transposase (Illumina; 20034197), 0.33X PBS, 0.1% Tween-20, 0.01% digitonin) and incubated at 37°C for 30 minutes with 1000 RPM mixing. .. After undergoing purification with the Zymo DNA Clean & Concentrator-5 kit (Zymo Research; D4003), samples were eluted in 21μl of elution buffer, and combined with 2.5μl of 25μM i5 primer, 2.5μl of 25μM i7 primer ( Supplementary Table 7 ), and 25μl 2X NEBNext High-Fidelity 2X PCR Master Mix (NEB; M0541S).

Article Title: MYC enhancer invasion promotes prognostic cancer type-specific gene programs through an epigenetic switch
Article Snippet: .. Nuclei were pelleted and resuspended in 50μL transposition mixture (17.75μL PBS, 5uL Nuclease Free Water, 25uL TD 2x reaction buffer, 0.5uL 1% digitonin, 0.5uL 10% Tween-20, 1.25μL TDE1 (Illumina, 20034197)) and incubated at 37°C in a thermomixer at 1000RPM for 30min. .. DNA was cleaned using Qiagen PCR Purification kit before PCR amplification for 10 cycles using Illumina index primers, 25μL Q5 High Fidelity 2x master mix (New England BioLabs, M0492) in a final volume of 50μL.

Article Title: Epigenetic priming of embryonic enhancer elements coordinates developmental gene networks
Article Snippet: .. The pellet was then resuspended in 50μl of transposition mixture (1X Illumina Tagment DNA Buffer and 100nM Illumina TDE1 Tagment DNA Enzyme transposase (Illumina; 20034197), 0.33X DPBS, 0.1% Tween-20, 0.01% digitonin) and incubated at 37°C for 30 min with 1000 RPM mixing. .. After undergoing purification with the Zymo DNA Clean & Concentrator-5 kit (Zymo Research; D4003), samples were eluted in 21μl of elution buffer, and combined with 2.5μl of 25μM i5 primer, 2.5μl of 25μM i7 primer, and 25μl 2X NEBNext High-Fidelity 2X PCR Master Mix (NEB; M0541S).

Article Title: Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fate
Article Snippet: .. The supernatant was removed and the nuclei pellet was resuspended in 50 μl transposition mixture [25 μl Tagment DNA Buffer (1× final, Illumina, 20034197), 2.5 μl Tagment Tn5 Transposase (100 nM final, Illumina, 20034197), 16.5 μl 1× PBS, 0.5 μl 1% Digitonin (0.01% final), 0.5 μl 10% tween 20 (0.1% final)] and incubated at 37°C for 30 min shaking at 1000 rpm. .. The tagmented DNA was purified with the Zymo DNA Clean and Concentrator-5 Kit (Zymo, D4014) and eluted in 21 μl Elution Buffer.

other:

Article Title: REST/NRSF Preserves muscle stem cell identity by repressing alternate cell fate
Article Snippet: The pellet was resuspended in 10 μL of transposition mixture (5 μL TD buffer, 3.2 μL PBS, 0.89 μL Tn5 (Illumina, 20034197), 0.1% Tween-20, 0.01% Digitonin and 0.75 μL nuclease free water) and incubated at 37 °C for 25 min for optimal tagmentation.

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